live or dye viability dye Search Results


99
Sartorius AG live cell imaging system
Live Cell Imaging System, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Sartorius AG m nucview caspase 3 7 apoptosis reagent
DAC and knockdown of CHD4 synergize in cell death induction, in correlation with reactivation of TSGs. (a) CHD4 siRNAs induce depletion of CHD4. RKO cells were transduced with scrambled (CONT1-2) or CHD4 siRNAs. CHD4 knockdown was verified by examining CHD4 protein levels, α-tubulin serves as a loading control. (b) Knockdown of CHD4 sensitizes RKO cells to DAC treatment. RKO cells from (a) were untreated or treated with indicated concentrations of DAC. Kinetic measures of the number of <t>caspase-3/7-positive</t> cells, recorded over time and plotted as fluorescent objects, divided by confluence are shown. n = 3 wells per data point shown, error bars denote s.d. (c, d) Knockdown of CHD4 induces synthetic sickness lethality with DNMTi in association with TSG reactivation. RKO cells from (a, b) were untreated or treated with 250 nm DAC and analyzed for caspase 3 and 7 activation by time-lapse microscopy after 140 h (c) and analyzed for TSG reactivation by QRT-PCR (d). Error bars denote s.d.
M Nucview Caspase 3 7 Apoptosis Reagent, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Rad itaq sybr green with rox
DAC and knockdown of CHD4 synergize in cell death induction, in correlation with reactivation of TSGs. (a) CHD4 siRNAs induce depletion of CHD4. RKO cells were transduced with scrambled (CONT1-2) or CHD4 siRNAs. CHD4 knockdown was verified by examining CHD4 protein levels, α-tubulin serves as a loading control. (b) Knockdown of CHD4 sensitizes RKO cells to DAC treatment. RKO cells from (a) were untreated or treated with indicated concentrations of DAC. Kinetic measures of the number of <t>caspase-3/7-positive</t> cells, recorded over time and plotted as fluorescent objects, divided by confluence are shown. n = 3 wells per data point shown, error bars denote s.d. (c, d) Knockdown of CHD4 induces synthetic sickness lethality with DNMTi in association with TSG reactivation. RKO cells from (a, b) were untreated or treated with 250 nm DAC and analyzed for caspase 3 and 7 activation by time-lapse microscopy after 140 h (c) and analyzed for TSG reactivation by QRT-PCR (d). Error bars denote s.d.
Itaq Sybr Green With Rox, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Rad protein assay dye reagent concentrate
DAC and knockdown of CHD4 synergize in cell death induction, in correlation with reactivation of TSGs. (a) CHD4 siRNAs induce depletion of CHD4. RKO cells were transduced with scrambled (CONT1-2) or CHD4 siRNAs. CHD4 knockdown was verified by examining CHD4 protein levels, α-tubulin serves as a loading control. (b) Knockdown of CHD4 sensitizes RKO cells to DAC treatment. RKO cells from (a) were untreated or treated with indicated concentrations of DAC. Kinetic measures of the number of <t>caspase-3/7-positive</t> cells, recorded over time and plotted as fluorescent objects, divided by confluence are shown. n = 3 wells per data point shown, error bars denote s.d. (c, d) Knockdown of CHD4 induces synthetic sickness lethality with DNMTi in association with TSG reactivation. RKO cells from (a, b) were untreated or treated with 250 nm DAC and analyzed for caspase 3 and 7 activation by time-lapse microscopy after 140 h (c) and analyzed for TSG reactivation by QRT-PCR (d). Error bars denote s.d.
Protein Assay Dye Reagent Concentrate, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher orange loading dye
DAC and knockdown of CHD4 synergize in cell death induction, in correlation with reactivation of TSGs. (a) CHD4 siRNAs induce depletion of CHD4. RKO cells were transduced with scrambled (CONT1-2) or CHD4 siRNAs. CHD4 knockdown was verified by examining CHD4 protein levels, α-tubulin serves as a loading control. (b) Knockdown of CHD4 sensitizes RKO cells to DAC treatment. RKO cells from (a) were untreated or treated with indicated concentrations of DAC. Kinetic measures of the number of <t>caspase-3/7-positive</t> cells, recorded over time and plotted as fluorescent objects, divided by confluence are shown. n = 3 wells per data point shown, error bars denote s.d. (c, d) Knockdown of CHD4 induces synthetic sickness lethality with DNMTi in association with TSG reactivation. RKO cells from (a, b) were untreated or treated with 250 nm DAC and analyzed for caspase 3 and 7 activation by time-lapse microscopy after 140 h (c) and analyzed for TSG reactivation by QRT-PCR (d). Error bars denote s.d.
Orange Loading Dye, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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New England Biolabs w v sds new england biolabs b7703s
DAC and knockdown of CHD4 synergize in cell death induction, in correlation with reactivation of TSGs. (a) CHD4 siRNAs induce depletion of CHD4. RKO cells were transduced with scrambled (CONT1-2) or CHD4 siRNAs. CHD4 knockdown was verified by examining CHD4 protein levels, α-tubulin serves as a loading control. (b) Knockdown of CHD4 sensitizes RKO cells to DAC treatment. RKO cells from (a) were untreated or treated with indicated concentrations of DAC. Kinetic measures of the number of <t>caspase-3/7-positive</t> cells, recorded over time and plotted as fluorescent objects, divided by confluence are shown. n = 3 wells per data point shown, error bars denote s.d. (c, d) Knockdown of CHD4 induces synthetic sickness lethality with DNMTi in association with TSG reactivation. RKO cells from (a, b) were untreated or treated with 250 nm DAC and analyzed for caspase 3 and 7 activation by time-lapse microscopy after 140 h (c) and analyzed for TSG reactivation by QRT-PCR (d). Error bars denote s.d.
W V Sds New England Biolabs B7703s, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Rad quick start bradford dye reagent
DAC and knockdown of CHD4 synergize in cell death induction, in correlation with reactivation of TSGs. (a) CHD4 siRNAs induce depletion of CHD4. RKO cells were transduced with scrambled (CONT1-2) or CHD4 siRNAs. CHD4 knockdown was verified by examining CHD4 protein levels, α-tubulin serves as a loading control. (b) Knockdown of CHD4 sensitizes RKO cells to DAC treatment. RKO cells from (a) were untreated or treated with indicated concentrations of DAC. Kinetic measures of the number of <t>caspase-3/7-positive</t> cells, recorded over time and plotted as fluorescent objects, divided by confluence are shown. n = 3 wells per data point shown, error bars denote s.d. (c, d) Knockdown of CHD4 induces synthetic sickness lethality with DNMTi in association with TSG reactivation. RKO cells from (a, b) were untreated or treated with 250 nm DAC and analyzed for caspase 3 and 7 activation by time-lapse microscopy after 140 h (c) and analyzed for TSG reactivation by QRT-PCR (d). Error bars denote s.d.
Quick Start Bradford Dye Reagent, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Valiant Co Ltd taq polymerase
DAC and knockdown of CHD4 synergize in cell death induction, in correlation with reactivation of TSGs. (a) CHD4 siRNAs induce depletion of CHD4. RKO cells were transduced with scrambled (CONT1-2) or CHD4 siRNAs. CHD4 knockdown was verified by examining CHD4 protein levels, α-tubulin serves as a loading control. (b) Knockdown of CHD4 sensitizes RKO cells to DAC treatment. RKO cells from (a) were untreated or treated with indicated concentrations of DAC. Kinetic measures of the number of <t>caspase-3/7-positive</t> cells, recorded over time and plotted as fluorescent objects, divided by confluence are shown. n = 3 wells per data point shown, error bars denote s.d. (c, d) Knockdown of CHD4 induces synthetic sickness lethality with DNMTi in association with TSG reactivation. RKO cells from (a, b) were untreated or treated with 250 nm DAC and analyzed for caspase 3 and 7 activation by time-lapse microscopy after 140 h (c) and analyzed for TSG reactivation by QRT-PCR (d). Error bars denote s.d.
Taq Polymerase, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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New England Biolabs gel electrophoresis gel loading dye
DAC and knockdown of CHD4 synergize in cell death induction, in correlation with reactivation of TSGs. (a) CHD4 siRNAs induce depletion of CHD4. RKO cells were transduced with scrambled (CONT1-2) or CHD4 siRNAs. CHD4 knockdown was verified by examining CHD4 protein levels, α-tubulin serves as a loading control. (b) Knockdown of CHD4 sensitizes RKO cells to DAC treatment. RKO cells from (a) were untreated or treated with indicated concentrations of DAC. Kinetic measures of the number of <t>caspase-3/7-positive</t> cells, recorded over time and plotted as fluorescent objects, divided by confluence are shown. n = 3 wells per data point shown, error bars denote s.d. (c, d) Knockdown of CHD4 induces synthetic sickness lethality with DNMTi in association with TSG reactivation. RKO cells from (a, b) were untreated or treated with 250 nm DAC and analyzed for caspase 3 and 7 activation by time-lapse microscopy after 140 h (c) and analyzed for TSG reactivation by QRT-PCR (d). Error bars denote s.d.
Gel Electrophoresis Gel Loading Dye, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fluidigm dna
DAC and knockdown of CHD4 synergize in cell death induction, in correlation with reactivation of TSGs. (a) CHD4 siRNAs induce depletion of CHD4. RKO cells were transduced with scrambled (CONT1-2) or CHD4 siRNAs. CHD4 knockdown was verified by examining CHD4 protein levels, α-tubulin serves as a loading control. (b) Knockdown of CHD4 sensitizes RKO cells to DAC treatment. RKO cells from (a) were untreated or treated with indicated concentrations of DAC. Kinetic measures of the number of <t>caspase-3/7-positive</t> cells, recorded over time and plotted as fluorescent objects, divided by confluence are shown. n = 3 wells per data point shown, error bars denote s.d. (c, d) Knockdown of CHD4 induces synthetic sickness lethality with DNMTi in association with TSG reactivation. RKO cells from (a, b) were untreated or treated with 250 nm DAC and analyzed for caspase 3 and 7 activation by time-lapse microscopy after 140 h (c) and analyzed for TSG reactivation by QRT-PCR (d). Error bars denote s.d.
Dna, supplied by fluidigm, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cytek Biosciences ghost dye red 780
DAC and knockdown of CHD4 synergize in cell death induction, in correlation with reactivation of TSGs. (a) CHD4 siRNAs induce depletion of CHD4. RKO cells were transduced with scrambled (CONT1-2) or CHD4 siRNAs. CHD4 knockdown was verified by examining CHD4 protein levels, α-tubulin serves as a loading control. (b) Knockdown of CHD4 sensitizes RKO cells to DAC treatment. RKO cells from (a) were untreated or treated with indicated concentrations of DAC. Kinetic measures of the number of <t>caspase-3/7-positive</t> cells, recorded over time and plotted as fluorescent objects, divided by confluence are shown. n = 3 wells per data point shown, error bars denote s.d. (c, d) Knockdown of CHD4 induces synthetic sickness lethality with DNMTi in association with TSG reactivation. RKO cells from (a, b) were untreated or treated with 250 nm DAC and analyzed for caspase 3 and 7 activation by time-lapse microscopy after 140 h (c) and analyzed for TSG reactivation by QRT-PCR (d). Error bars denote s.d.
Ghost Dye Red 780, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Vazyme Biotech Co 2x taq master mix
DAC and knockdown of CHD4 synergize in cell death induction, in correlation with reactivation of TSGs. (a) CHD4 siRNAs induce depletion of CHD4. RKO cells were transduced with scrambled (CONT1-2) or CHD4 siRNAs. CHD4 knockdown was verified by examining CHD4 protein levels, α-tubulin serves as a loading control. (b) Knockdown of CHD4 sensitizes RKO cells to DAC treatment. RKO cells from (a) were untreated or treated with indicated concentrations of DAC. Kinetic measures of the number of <t>caspase-3/7-positive</t> cells, recorded over time and plotted as fluorescent objects, divided by confluence are shown. n = 3 wells per data point shown, error bars denote s.d. (c, d) Knockdown of CHD4 induces synthetic sickness lethality with DNMTi in association with TSG reactivation. RKO cells from (a, b) were untreated or treated with 250 nm DAC and analyzed for caspase 3 and 7 activation by time-lapse microscopy after 140 h (c) and analyzed for TSG reactivation by QRT-PCR (d). Error bars denote s.d.
2x Taq Master Mix, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


DAC and knockdown of CHD4 synergize in cell death induction, in correlation with reactivation of TSGs. (a) CHD4 siRNAs induce depletion of CHD4. RKO cells were transduced with scrambled (CONT1-2) or CHD4 siRNAs. CHD4 knockdown was verified by examining CHD4 protein levels, α-tubulin serves as a loading control. (b) Knockdown of CHD4 sensitizes RKO cells to DAC treatment. RKO cells from (a) were untreated or treated with indicated concentrations of DAC. Kinetic measures of the number of caspase-3/7-positive cells, recorded over time and plotted as fluorescent objects, divided by confluence are shown. n = 3 wells per data point shown, error bars denote s.d. (c, d) Knockdown of CHD4 induces synthetic sickness lethality with DNMTi in association with TSG reactivation. RKO cells from (a, b) were untreated or treated with 250 nm DAC and analyzed for caspase 3 and 7 activation by time-lapse microscopy after 140 h (c) and analyzed for TSG reactivation by QRT-PCR (d). Error bars denote s.d.

Journal: Oncogene

Article Title: The NuRD complex cooperates with DNMTs to maintain silencing of key colorectal tumor suppressor genes

doi: 10.1038/onc.2013.178

Figure Lengend Snippet: DAC and knockdown of CHD4 synergize in cell death induction, in correlation with reactivation of TSGs. (a) CHD4 siRNAs induce depletion of CHD4. RKO cells were transduced with scrambled (CONT1-2) or CHD4 siRNAs. CHD4 knockdown was verified by examining CHD4 protein levels, α-tubulin serves as a loading control. (b) Knockdown of CHD4 sensitizes RKO cells to DAC treatment. RKO cells from (a) were untreated or treated with indicated concentrations of DAC. Kinetic measures of the number of caspase-3/7-positive cells, recorded over time and plotted as fluorescent objects, divided by confluence are shown. n = 3 wells per data point shown, error bars denote s.d. (c, d) Knockdown of CHD4 induces synthetic sickness lethality with DNMTi in association with TSG reactivation. RKO cells from (a, b) were untreated or treated with 250 nm DAC and analyzed for caspase 3 and 7 activation by time-lapse microscopy after 140 h (c) and analyzed for TSG reactivation by QRT-PCR (d). Error bars denote s.d.

Article Snippet: DAC (15–1000 n m ) and 1:1000 CellPlayer 5 m m NucView Caspase-3/7 Apoptosis reagent (Essen BioScience, Ann Arbor, MI, USA) were added every subsequent day when medium was refreshed.

Techniques: Transduction, Activation Assay, Time-lapse Microscopy, Quantitative RT-PCR