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Image Search Results
Journal: Oncogene
Article Title: The NuRD complex cooperates with DNMTs to maintain silencing of key colorectal tumor suppressor genes
doi: 10.1038/onc.2013.178
Figure Lengend Snippet: DAC and knockdown of CHD4 synergize in cell death induction, in correlation with reactivation of TSGs. (a) CHD4 siRNAs induce depletion of CHD4. RKO cells were transduced with scrambled (CONT1-2) or CHD4 siRNAs. CHD4 knockdown was verified by examining CHD4 protein levels, α-tubulin serves as a loading control. (b) Knockdown of CHD4 sensitizes RKO cells to DAC treatment. RKO cells from (a) were untreated or treated with indicated concentrations of DAC. Kinetic measures of the number of caspase-3/7-positive cells, recorded over time and plotted as fluorescent objects, divided by confluence are shown. n = 3 wells per data point shown, error bars denote s.d. (c, d) Knockdown of CHD4 induces synthetic sickness lethality with DNMTi in association with TSG reactivation. RKO cells from (a, b) were untreated or treated with 250 nm DAC and analyzed for caspase 3 and 7 activation by time-lapse microscopy after 140 h (c) and analyzed for TSG reactivation by QRT-PCR (d). Error bars denote s.d.
Article Snippet: DAC (15–1000 n m ) and 1:1000 CellPlayer 5 m
Techniques: Transduction, Activation Assay, Time-lapse Microscopy, Quantitative RT-PCR